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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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30 from 658 pages
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Anti-GOAT IgG (H&L), DyLight(TM) 649 Conjugated
Anti-GOAT IgG (H&L), DyLight(TM) 649 Conjugated

Item number: 605-443-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Rabbit
Species reactivity: goat
From 171.00€ *
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Anti-GOAT IgG (H&L), DyLight(TM) 549 Conjugated
Anti-GOAT IgG (H&L), DyLight(TM) 549 Conjugated

Item number: 605-742-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: goat
From 177.00€ *
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iFluor(TM) 555 goat anti-rabbit IgG (H+L)
iFluor(TM) 555 goat anti-rabbit IgG (H+L)

Item number: ABD-16620

AAT Bioquest
Ex: 559 nm. Em: 569 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF, ICC
Host: Goat
Species reactivity: rabbit
From 114.00€ *
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Anti-RAT IgG (H&L), DyLight(TM) 405 Conjugated
Anti-RAT IgG (H&L), DyLight(TM) 405 Conjugated

Item number: 612-446-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Rabbit
Species reactivity: rat
From 171.00€ *
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Anti-Human IgG Fc [JDC-10] (HRP) [Mouse]
Anti-Human IgG Fc [JDC-10] (HRP) [Mouse]

Item number: ARG23933.500

Arigo
Application: ELISA, FC, FLISA, IHC (paraffin), WB
Host: Mouse
Species reactivity: human, chimpanzee, rhesus monkey
427.00€ *
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iFluor(TM) 647 goat anti-rabbit IgG (H+L)
iFluor(TM) 647 goat anti-rabbit IgG (H+L)

Item number: ABD-16809

AAT Bioquest
Ex: 654 nm. Em: 674 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF
Host: Goat
Species reactivity: rabbit
From 114.00€ *
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iFluor(TM) 790 goat anti-mouse IgG (H+L)
iFluor(TM) 790 goat anti-mouse IgG (H+L)

Item number: ABD-16750

AAT Bioquest
Ex: 782 nm. Em: 811 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF
Host: Goat
Species reactivity: mouse
From 114.00€ *
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FITC goat anti-rabbit IgG (H+L)
FITC goat anti-rabbit IgG (H+L)

Item number: ABD-16868

AAT Bioquest
Ex: 492 nm. Em: 515 nm. Soluble in: Water. AAT Bioquest's anti-mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. This FITC-labeled secondary antibody was prepared...
Application: IF
Host: Goat
Species reactivity: rabbit
218.00€ *
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Cy5(R) goat anti-mouse IgG (H+L)
Cy5(R) goat anti-mouse IgG (H+L)

Item number: ABD-16855

AAT Bioquest
Ex: 649 nm. Em: 665 nm. Soluble in: Water. AAT Bioquest's anti-mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Cy5-labeled secondary antibody was prepared...
Application: IF
Host: Goat
Species reactivity: mouse
218.00€ *
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Cy5(R) goat anti-rabbit IgG (H+L) *Cross Adsorbed*
Cy5(R) goat anti-rabbit IgG (H+L) *Cross Adsorbed*

Item number: ABD-16879

AAT Bioquest
Ex: 649 nm. Em: 665 nm. Soluble in: Water. AAT Bioquest's anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Cy5-labeled secondary antibody was prepared...
Application: IF
Host: Goat
Species reactivity: rabbit
291.00€ *
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Anti-IgA (human), clone Hisa43, R-PE conjugated
Anti-IgA (human), clone Hisa43, R-PE conjugated

Item number: ANC-137-050

Ancell
Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by...
Keywords: Anti-IGHA, Anti-Ig alpha chain C region
Application: FC, ELISA
Host: Mouse
Species reactivity: human
383.00€ *
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Anti-IgG (human), clone ICO-97, preservative free
Anti-IgG (human), clone ICO-97, preservative free

Item number: ANC-139-820

Ancell
Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by...
Application: FC, ELISA
Host: Mouse
Species reactivity: human
256.00€ *
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30 from 658 pages
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