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from to

Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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22 from 658 pages
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Anti-MOUSE IgG (H&L), DyLight(TM) 405 Conjugated
Anti-MOUSE IgG (H&L), DyLight(TM) 405 Conjugated

Item number: 610-446-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Rabbit
Species reactivity: mouse
From 171.00€ *
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Review
iFluor(TM) 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor(TM) 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*

Item number: ABD-16528

AAT Bioquest
Ex: 491 nm. Em: 514 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF, ICC
Host: Goat
Species reactivity: mouse
From 145.00€ *
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Anti-RABBIT IgG (H&L), DyLight(TM) 649 Conjugated
Anti-RABBIT IgG (H&L), DyLight(TM) 649 Conjugated

Item number: 611-143-002-0.5

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum,...
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: rabbit
From 171.00€ *
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Review
Anti-GOAT IgG (H&L), DyLight(TM) 549 Conjugated
Anti-GOAT IgG (H&L), DyLight(TM) 549 Conjugated

Item number: 605-442-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Rabbit
Species reactivity: goat
From 171.00€ *
Show details
Review
Anti-MOUSE IgG (H&L), DyLight(TM) 549 Conjugated
Anti-MOUSE IgG (H&L), DyLight(TM) 549 Conjugated

Item number: 610-442-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum,...
Application: FLISA, IFM, WB
Host: Rabbit
Species reactivity: mouse
From 171.00€ *
Show details
Review
iFluor(TM) 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor(TM) 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*

Item number: ABD-16587

AAT Bioquest
AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 790 goat anti-mouse...
Application: WB
Host: Goat
Species reactivity: mouse
From 145.00€ *
Show details
Review
Texas Red(R) goat anti-rabbit IgG (H+L)
Texas Red(R) goat anti-rabbit IgG (H+L)

Item number: ABD-16869

AAT Bioquest
Ex: 582 nm. Em: 602 nm. Soluble in: Water. AAT Bioquest's anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Texas Red-labeled secondary antibody was...
Application: IF
Host: Goat
Species reactivity: rabbit
218.00€ *
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iFluor(TM) 594 goat anti-rabbit IgG (H+L)
iFluor(TM) 594 goat anti-rabbit IgG (H+L)

Item number: ABD-16628

AAT Bioquest
Ex: 592 nm. Em: 614 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF, ICC
Host: Goat
Species reactivity: rabbit
From 114.00€ *
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Cy3(R) goat anti-rabbit IgG (H+L) *Cross Adsorbed*
Cy3(R) goat anti-rabbit IgG (H+L) *Cross Adsorbed*

Item number: ABD-16878

AAT Bioquest
Ex: 555 nm. Em: 565 nm. Soluble in: Water. AAT Bioquest's anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Cy3-labeled secondary antibody was prepared...
Application: IF
Host: Goat
Species reactivity: rabbit
291.00€ *
Show details
Review
iFluor(TM) 488 goat anti-mouse IgG (H+L)
iFluor(TM) 488 goat anti-mouse IgG (H+L)

Item number: ABD-16448

AAT Bioquest
Ex: 491 nm. Em: 514 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF, ICC
Host: Goat
Species reactivity: mouse
From 114.00€ *
Show details
Review
iFluor(TM) 750 goat anti-mouse IgG (H+L)
iFluor(TM) 750 goat anti-mouse IgG (H+L)

Item number: ABD-16748

AAT Bioquest
Ex: 753 nm. Em: 779 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF
Host: Goat
Species reactivity: mouse
From 114.00€ *
Show details
Review
iFluor(TM) 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor(TM) 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*

Item number: ABD-16524

AAT Bioquest
Ex: 401 nm. Em: 420 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF, ICC
Host: Goat
Species reactivity: mouse
From 145.00€ *
Show details
Review
22 from 658 pages
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