Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-Rabbit IgG (H&L) [Donkey] Alkaline Phosphatase conjugated
Anti-Rabbit IgG (H&L) [Donkey] Alkaline Phosphatase...

Item number: 611-7502

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: rabbit
292.00€ *
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Anti-Rat IgG (H&L) [Goat] (Min X Bv Ch Gt Gp Ham Hs Hu Ms Rb & Sh serum proteins)
Anti-Rat IgG (H&L) [Goat] (Min X Bv Ch Gt Gp Ham Hs Hu Ms...

Item number: 612-101-120

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rat
252.00€ *
Review
Anti-Rat IgG (H&L), DyLight(TM) 800 conjugated
Anti-Rat IgG (H&L), DyLight(TM) 800 conjugated

Item number: 612-145-120

Maximally cross-adsorbed and affinity purified goat anti-rat IgG (H+L) conjugated to DyLight(TM) 800. High fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: Rat
177.00€ *
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Anti-Rat IgG (H&L) [Donkey] Peroxidase conjugated
Anti-Rat IgG (H&L) [Donkey] Peroxidase conjugated

Item number: 612-703-002

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: rat
252.00€ *
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Anti-Rat IgG (H&L) [Donkey] (Min X Bv Ch Gt Gp Ham Hs Hu Ms Rb & Sh serum proteins) Biotin conjugate
Anti-Rat IgG (H&L) [Donkey] (Min X Bv Ch Gt Gp Ham Hs Hu...

Item number: 612-706-120

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: rat
361.00€ *
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Anti-Sheep IgG (H&L) (Min X Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins), DyLight 800 conjugated
Anti-Sheep IgG (H&L) (Min X Ch GP Ham Hs Hu Ms Rb & Rt...

Item number: 613-745-168

DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: Sheep
177.00€ *
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Anti-Monkey IgM (Mu Chain) [Goat]
Anti-Monkey IgM (Mu Chain) [Goat]

Item number: 617-101-007

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: monkey
292.00€ *
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Anti-Armenian Hamster IgG (H&L) [Goat] Biotin conjugated
Anti-Armenian Hamster IgG (H&L) [Goat] Biotin conjugated

Item number: 619-106-002

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: armenian hamster
292.00€ *
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Anti-Golden Syrian & Armenian Hamster IgG (H&L) (Min X Mouse and Rat Serum Proteins), DyLight 488 co
Anti-Golden Syrian & Armenian Hamster IgG (H&L) (Min X...

Item number: 620-141-440

DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: golden syrian & armenian hamster
177.00€ *
Review
Anti-Human IgG F(c) [Goat] (Min X Bv Hs Ms & Rt serum proteins) Alkaline Phosphatase conjugated F(ab
Anti-Human IgG F(c) [Goat] (Min X Bv Hs Ms & Rt serum...

Item number: 709-1517

Format - F(ab')2, affinity purified
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: human
426.00€ *
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Anti-Human IgG (H&L) [Goat] Fab fragment
Anti-Human IgG (H&L) [Goat] Fab fragment

Item number: 809-1102

Format - Fab fragment
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: human
252.00€ *
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Anti-Chicken IgG (H&L), DyLight(TM) 680 Conjugated
Anti-Chicken IgG (H&L), DyLight(TM) 680 Conjugated

Item number: 603-144-002

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by...
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: chicken
From 171.00€ *
Review
21 from 658 pages