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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-Mouse IgG (H&L) [DONKEY], DyLight 800 conjugated
Anti-Mouse IgG (H&L) [DONKEY], DyLight 800 conjugated

Item number: 610-745-002

Rockland
DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: mouse
171.00€ *
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Anti-Rabbit IgG [Goat] (Minimum cross-reactivity) Peroxidase conjugated
Anti-Rabbit IgG [Goat] (Minimum cross-reactivity)...

Item number: 611-103-122

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rabbit
431.00€ *
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Anti-Rabbit IgG F(c) [Goat]
Anti-Rabbit IgG F(c) [Goat]

Item number: 611-1103

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rabbit
228.00€ *
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Anti-Rabbit IgG (H&L) (Goat), ATTO 425 conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Prot
Anti-Rabbit IgG (H&L) (Goat), ATTO 425 conjugated (Min X...

Item number: 611-151-122S

Rockland
Format - Whole IgG
Keywords: AT425, ATTO 425, ATTO-TEC 425
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: rabbit
From 194.00€ *
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Anti-Rabbit IgG (H&L) [Mouse] (Min X Hu, Gt, Ms serum proteins)
Anti-Rabbit IgG (H&L) [Mouse] (Min X Hu, Gt, Ms serum...

Item number: 611-301-122

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Mouse
Species reactivity: rabbit
240.00€ *
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Anti-Rat IgG (H&L) [Donkey] (Min X Bv Ch Gt Gp Ham Hs Hu Ms Rb & Sh serum proteins) Peroxidase conju
Anti-Rat IgG (H&L) [Donkey] (Min X Bv Ch Gt Gp Ham Hs Hu...

Item number: 612-703-120

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: rat
418.00€ *
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Anti-Rat IgG (H&L) [Chicken] Peroxidase conjugated
Anti-Rat IgG (H&L) [Chicken] Peroxidase conjugated

Item number: 612-9302

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Chicken
Species reactivity: rat
325.00€ *
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Anti-Swine IgG (H&L) [Goat] Fluorescein conjugated
Anti-Swine IgG (H&L) [Goat] Fluorescein conjugated

Item number: 614-1202

Rockland
Format - Whole IgG
Application: FLISA, FC, IFM
Host: Goat
Species reactivity: swine
204.00€ *
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Anti-Rabbit IgG (H&L) [Goat] Min X Bv Hs Hu Ms Rt & Sh serum proteins Biotin conjugated F(ab')2 frag
Anti-Rabbit IgG (H&L) [Goat] Min X Bv Hs Hu Ms Rt & Sh...

Item number: 711-1622

Rockland
Format - F(ab')2, affinity purified
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rabbit
308.00€ *
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Anti-RAT IgG (H&L), DyLight(TM) 549 Conjugated
Anti-RAT IgG (H&L), DyLight(TM) 549 Conjugated

Item number: 612-142-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat...
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: rat
From 171.00€ *
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Anti-IgM Heavy Chain, clone rIGHM/2558
Anti-IgM Heavy Chain, clone rIGHM/2558

Item number: NSJ-V7868-20UG

NSJBio
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide. Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is...
Keywords: Anti-Ig mu chain C region, Anti-Ig mu chain C region OU, Anti-Ig mu chain C region BOT, Anti-Ig mu chain C region GAL,...
Application: FC, IF, IHC (paraffin)
Host: Mouse
Species reactivity: human
From 375.00€ *
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iFluor(TM) 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor(TM) 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

Item number: ABD-16843

AAT Bioquest
Ex: 782 nm. Em: 811 nm. Soluble in: Water. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555...
Application: IF
Host: Goat
Species reactivity: rabbit
From 145.00€ *
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13 from 661 pages
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