Anti-Human MGMT Antibody Pair

Product Description: O-6-methylguanine-DNA methyltransferase (MGMT) is a DNA repair enzyme that directly reverses alkylation damage at the O-6 position of guanine, preventing mutagenic mismatches during replication. This monomeric protein (21.7 kDa) employs a suicide mechanism, transferring alkyl groups to its active site cysteine residue (Cys145), irreversibly inactivating itself. Expressed ubiquitously, particularly in the liver and brain, MGMT safeguards genomic integrity by mitigating G:C to A:T mutations. In cancer, epigenetic silencing via promoter methylation reduces MGMT expression, a hallmark of gliomas, glioblastomas, and other malignancies. Tumors with methylated MGMT promoters exhibit heightened sensitivity to alkylating agents like temozolomide, as impaired repair leads to lethal DNA damage accumulation. Conversely, unmethylated promoters correlate with therapeutic resistance, making MGMT status a critical biomarker for predicting chemotherapy efficacy. Protein Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated [The Uniprot Consortium]