Anti-Human IFI16 Antibody Pair

Product Description: Interferon gamma inducible protein 16 (IFI16) is a protein encoded by the IFI16 gene in humans. It belongs to the HIN-200 family of cytokines and contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. IFI16 localizes to the nucleoplasm and nucleoli, interacts with p53, modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. It plays a crucial role in sensing intracellular DNA, a hallmark of virally infected cells, and has been linked to the death of HIV-infected helper CD4 T cells by pyroptosis. IFI16 can detect various forms of DNA, including double-stranded DNA, single-stranded DNA, and quadruplex DNA, and it has been implicated in the restriction of viral replication, including HSV-1 and HIV-1. Additionally, IFI16 has been shown to induce inflammation upon TLR4 binding when extracellularly released, acting as a damage-associated molecular pattern (DAMP). Protein Function: Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer , Isoform that specifically inhibits the AIM2 inflammasome (PubMed:30104205). Binds double-stranded DNA (dsDNA) in the cytoplasm, impeding its detection by AIM2 (PubMed:30104205). Also prevents the interaction between AIM2 and PYCARD/ASC via its interaction with AIM2, thereby inhibiting assembly of the AIM2 inflammasome (PubMed:30104205). This isoform also weakly induce production of type I interferon-beta (IFNB1) via its interaction with STING1 (PubMed:30104205) [The Uniprot Consortium]