Anti-Human CFAP45 Antibody Pair

Product Description: Cilia and flagella associated protein 45 (CFAP45) is a cytoskeletal protein integral to the axonemal structure of motile cilia and flagella, where it interacts with dynein ATPases and adenylate kinase to regulate adenine nucleotide homeostasis, particularly by binding AMP and facilitating microtubule sliding necessary for effective ciliary and flagellar beating. CFAP45 contains domains that enable stable three-dimensional folding and specific interactions with other proteins, contributing to its role in the axonemal module that controls motility. Deficiency of CFAP45 in humans and mice leads to a motile ciliopathy characterized by normal ciliary morphology but impaired function, manifesting clinically as situs inversus totalis and asthenospermia due to dyskinetic beating of nodal cilia and sperm flagella. Restoration of nucleotide balance with AMP or ADP can rescue the motility defect, highlighting CFAP45's essential role in maintaining proper ciliary and flagellar movement. Protein Function: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:36191189). It is an AMP-binding protein that may facilitate dynein ATPase-dependent ciliary and flagellar beating via adenine nucleotide homeostasis. May function as a donor of AMP to AK8 and hence promote ADP production (PubMed:33139725) [The Uniprot Consortium]