Anti-Human 53BP1 Antibody Pair

Product Description: Tumor protein p53 binding protein 1 (TP53BP1) is a crucial protein involved in DNA double-strand break repair, particularly in promoting non-homologous end joining (NHEJ) pathways. It interacts with damaged chromatin, recognizing specific epigenetic markers, and plays a role in telomere dynamics and class-switch recombination during antibody genesis. TP53BP1 forms a complex with RIF1, inhibiting DNA end resection and competing with BRCA1-CtIP to determine the repair pathway choice. The protein contains multiple functional domains and exists in three cellular pools: cytoplasmic, nuclear, and DNA repair foci. TP53BP1 also binds to the p53 protein, regulating cell cycle progression and proliferation. In terms of disease relevance, TP53BP1 is underexpressed in most cases of triple-negative breast cancer. Additionally, as individuals age, the recruitment of 53BP1 to double-strand breaks during the G1 phase becomes deficient, potentially contributing to the aging process through error-prone repair mechanisms. Protein Function: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:17190600, PubMed:21144835, PubMed:22553214, PubMed:23333306, PubMed:27153538, PubMed:28241136, PubMed:31135337, PubMed:37696958). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23333306, PubMed:23727112, PubMed:27153538, PubMed:31135337). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:17190600, PubMed:23760478, PubMed:27153538, PubMed:28241136). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112) [The Uniprot Consortium]