X-Gal

X-Gal is a chromogenic substrate for beta-galactosidase. Upon cleavage by beta-galactosidase, a blue pigment is released that can be used as a marker of beta-galactosidase activity. X-Gal has been used for the detection of lacZ activity in reporter assays and in the determination of protein-protein interactions.Formal Name: 5-bromo-4-chloro-1H-indol-3-yl-beta-D-galactopyranoside. CAS Number: 7240-90-6. Synonyms: BCIG, X-beta-Gal, 5-bromo-4-chloro-3-indolyl-beta-D-Galactopyranoside. Molecular Formula: C14H15BrClNO6. Formula Weight: 408.6. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml. lambdamax: 233, 292 nm. SMILES: O[C@H]1[C@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)O[C@H](CO)[C@H](O)[C@@H]1O. InChi Code: InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1. InChi Key: OPIFSICVWOWJMJ-AEOCFKNESA-N.