Speedy Supreme NZYTaq 2x Green Master Mix

Speedy Supreme NZYTaq 2x Green Master Mix is a premixed ready-to-use solution containing Speedy Supreme NZYTaq DNA polymerase (MB390), a recombinant modified form of Taq DNA polymerase that combines hot-start like PCR capacity and a fast polymerization reaction. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity and sensitivity of DNA amplification. The functional activity of the enzyme is restored during a short 5- minute incubation step at 95°C. The enzyme only requires 5 seconds to successfully amplify a DNA fragment of 1 kb. The enzyme retains its speed when amplifying fragments up to around 2 kb. Successful amplification of higher DNA fragments up to 6 kb in size can be reached using a 10 sec/kb extension step. Faster PCR can be further achieved by increasing the primers melting temperature, which increases PCR annealing temperature, thus allowing combining the annealing and extension PCR steps during PCR cycling (see below). The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Speedy Supreme NZYTaq 2x Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. Speedy Supreme NZYTaq 2x Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes Speedy Supreme NZYTaq 2x Colourless Master Mix (MB392) or purifying the PCR product using NZYGelpure (MB011) before performing any other protocol. Speedy Supreme NZYTaq DNA polymerase lacks 3'->5' exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech's NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137). Components: Speedy Supreme NZYTaq 2x Green Master Mix (0.2 U/µL). Shipping Conditions: Blue Ice. Storage Conditions: -85°C to -15°C. Application: Colony PCR, Mouse Genotyping, Powerful PCR, TA Cloning. Features: Fast, Hot-start. Product Length: 0-6 kb. Format: Mastermix. Product Category: End-Point PCR. Concentration: 0.2 U/µL. Hot Start: Yes