Proteinase K Reagent(10 mg/mL)

Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on. Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity. The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0. The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55 °C. Activity >30U/mg. DNase Free. Buffer PBS with 10mM Tris-HCl(pH 7.5),5mM CaCl 2 and 50% glycerin. RNase Free.