One-step NZYSpeedy qPCR Probe kit, ROX plus

One-step NZYSpeedy RT-qPCR Probe kit, ROX plus is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZYSpeedy qPCR Probe master mix developed for fast PCR, provided as a 2x reaction mixture, which contains all components necessary for real-time PCR, including dNTPs, stabilizers, and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA. It was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in the One-step NZYSpeedy RT-qPCR Probe kit, ROX plus, including buffer chemistry and a polymerase with hotstart-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results. Components: * One-step NZYSpeedy qPCR Probe master mix (2x), ROX plus * NZYRT mix * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85°C to -15°C. Starting Material: RNA. Features: Fast, Hot-start. Detection Method: Probe. Reference Dye: ROX plus. Format: Kit, Liquid. Product Category: Real-Time PCR. Compatibility: AB(TM) 7000, AB(TM) 7300, AB(TM) 7700, AB(TM) 7900 Fast, AB(TM) 7900 HT, AB(TM) 7900 HT Fast, AB(TM) StepOne Plus(TM), AB(TM) StepOne(TM). Protocol Time: Approx. 60 min. Hot Start: Yes