One-step NZYSpeedy qPCR Probe kit, ROX

One-step NZYSpeedy RT-qPCR Probe kit, ROX is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZYSpeedy qPCR Probe master mix developed for fast PCR, provided as a 2x reaction mixture, which contains all components necessary for real-time PCR, including dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA. It was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in the One-step NZYSpeedy RT-qPCR Probe kit, ROX, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results. Components: * One-step NZYSpeedy qPCR Probe master mix (2x), ROX * NZYRT mix * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85°C to -15°C. Starting Material: RNA. Features: Fast, Hot-start. Detection Method: Probe. Reference Dye: ROX. Format: Kit, Liquid. Product Category: Real-Time PCR. Compatibility: AB(TM) 7500, AB(TM) 7500 Fast, AB(TM) QuantStudio(TM) 12K, AB(TM) QuantStudio(TM) 3, AB(TM) QuantStudio(TM) 5, AB(TM) QuantStudio(TM) 6, AB(TM) QuantStudio(TM) 7, AB(TM) QuantStudio(TM) ViiA7(TM), Agilent AriaMx, Agilent Mx3000P(TM), Agilent Mx3005P(TM), Agilent Mx4000(TM), Fluidigm(R) Biomark(TM). Protocol Time: Approx. 60 min. Hot Start: Yes