NZY Bacterial Cell Lysis Buffer

NZY Bacterial Cell Lysis Buffer is an innovative product developed by NZYTech for the gentle disruption of Escherichia coli cell wall, generating a homogeneous cell-free extract. It provides a rapid and cost-effective alternative to harsh chemicals or mechanical procedures, such as French Press or sonication, to release recombinant and native proteins without denaturation. This extraction reagent is a Tris-buffered formulation (pH 7.5) with lysozyme and DNase I provided separately. The addition of these enzymes allows a most efficient extraction.In , protein extraction efficiencies are strain-dependent. NZY Bacterial Cell Lysis Buffer can be used to disrupt most common bacterial host strains and it is especially efficient with BL21 strain and its derivatives. The use of pLysS or pLysE hosts enhances protein extraction efficacies. Components: * NZY Bacterial Cell Lysis Buffer * Lysozyme (at 50 mg/mL) * DNase I (at 2 mg/mL). Shipping Conditions: Room Temperature. Storage Conditions: 15 °C to 25 °C. Application: Cell Lysis. Product Category: Cell preparation & Growth