Multiplex PCR NZYTaq 2x Green Master Mix

Multiplex PCR NZYTaq 2x Green Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification. In addition, reactions assembled with Multiplex PCR NZYTaq 2x Green Master Mix may be directly loaded onto agarose gels, which simplifies the workflow. There are two dyes (blue and yellow) in the mix that allow monitoring the progress of electrophoresis. Multiplex PCR NZYTaq 2x Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes - Multiplex PCR NZYTaq 2x Colourless Master Mix (MB336) - or purifying the PCR product using NZYGelpure (MB011) before performing any other protocol. Extension time: 30 sec/kb at 72 °C 3'->5' activity: no Product overhang: 3'-A. Components: Multiplex PCR NZYTaq 2x Green Master Mix (0.2 U/µL). Shipping Conditions: Blue Ice. Storage Conditions: -85°C to -15°C. Application: Multiplex PCR, TA Cloning, Typing/Detection. Features: Hot-start, Multiplex. Product Length: 0-3 kb. Format: Mastermix. Product Category: End-Point PCR. Concentration: 0.2 U/µL. Hot Start: Yes