Lyo NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix (2x)

Lyo NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix is an optimized and highly efficient freeze-dried reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimizes the risk of errors and contaminations. The master mix, formulated as a 2x reaction mixture, contains all components necessary for both cDNA synthesis and multiplex qPCR amplification (including enzymes, dNTPs, stabilizers and enhancers). Depending on the real-time thermal cycler used, up to 5-6 targets can be quantified simultaneously in the same well or tube. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes mixed in an optimized proportion specifically designed for the highest-performance. This master mix does not contain ROX and it was specifically developed for probe-detection technology. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the section "ROX reference dye" (ROX not provided). Components: * Lyo NZYSupreme Multiplex One-step qPCR Probe Master Mix (2x) * Multiplex RT-qPCR master mix reconstitution buffer. Shipping Conditions: Room Temperature. Storage Conditions: -85 °C to -15 °C. Starting Material: RNA. Features: Dual Hot-start, Multiplex, Ultra-sensitive. Detection Method: Probe. Reference Dye: No ROX. Format: Lyophilized, Mastermix. Product Category: Real-Time PCR. Compatibility: Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4(TM), Bio-Rad iCycler(TM) IQ, Bio-Rad iCycler(TM) IQ 4, Bio-Rad iCycler(TM) IQ 5, Bio-Rad iCycler(TM) MiniOpticon, Bio-Rad MyiQ(TM), Bio-Rad Opticon2(TM), Bio-Rad Opticon(TM), Bioer Gene Touch 96, Illumina Eco(TM), Roche LightCycler(R) 480, Roche LightCycler(R) 96, Roche LightCycler(R) Nano, Rotor-Gene(TM) 3000, Rotor-Gene(TM) 6000, Rotor-Gene(TM) Q. Concentration: 2x. Protocol Time: Approx. 60 min. Hot Start: Yes