Dulbecco's Phosphate Buffered Saline (PBS) (10X) (Powder)

Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process. Also Available: D9819: Dulbecco's Phosphate Buffered Saline (PBS) (Powder), D9820: Dulbecco's Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder), D9820-04: Dulbecco's Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder), Dulbecco's Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder), D9821: Dulbecco's Phosphate Buffered Saline (PBS) (10X) (Powder), D9822: Dulbecco's Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder), D9823: Dulbecco's Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder), Appearance: White, homogeneous, free flowing powder , Solubility (1X): Colorless, clear, complete., Some precipitate may be observed as a 10X due to excess concentration of salts. Sterile filter after solubilization. pH: As Reported , Endotoxin: <1EU/ml, Formulation: Components g/L, Calcium Chloride2H2O 1.325, Magnesium Chloride6H2O 1.00, Potassium Chloride 2.00, Potassium Phosphate Mono 2.00, Sodium Chloride 80.00, Sodium Phosphate Dibasic 11.50, Total: 97.8g/L, Directions: To make a 10X solution:, Dissolve 97.8g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.. Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization. , USB recommends the preparation of this product as a 1X solution. To make a 1X solution: Dissolve 9.78g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time., , Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.