Agarase, beta (b-Agarase)

beta-Agarase has a molecular weight of 32kD and hydrolyzes the 1,4-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. It may be used to investigate the structure of different galactans and as an enzymatic reagent for degrading agarose used as a gel medium in electrophoresis. This allows the isolation of oligonucleotides and DNA fragments from gels prepared with low melting point agarose. Synonyms: beta-Agarase, Agarose 4-glycanohydrolase, E.C. 3.2.1.81, CAS No: 37288-57-6, Source: Pseudomonas atlantica, Appearance: Supplied as a off-white to light yellow or light tan lyophilized powder, Protein: 10-30%, Activity (U/mgS) >0units/mg solid, Specific Activity: >5,000U/mg protein, Unit Definition: One unit will produce 1.0ug of reducing sugar (measured as D-galactose) from agar per minute at pH 6.0 and 40°C. Applications: Quantitative recovery of DNA or RNA from low melting point agarose gels. The recovered nucleic acids can be subjected to many further manipulations such as restriction endonuclease digestion, ligation, labeling, sequencing, amplification, etc. Storage and Stability: Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.