50X Tris-Acetate-EDTA (TAE)

Tris-Acetate-EDTA buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiplemolecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.