20X SSC pH 7.0

20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays). SSC is used to help control the stringency of hybridization in these steps.