DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioAssay (Supplied with 6x Loading Dye Solution)

DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50., Note: The bright bands at 500 and 250bp serve as the reference. Concentration: 0.5mg DNA/ml, Storage Buffer: 10mM Tris-HCl (pH 7.6), 1mM EDTA, 6X Loading Dye Solution: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Quality Control: Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns. Quantity of DNA in Band: Fragment (bp) % ng/0.5ug DNA, 1000 6 30, 900 6 30, 800 6 30 , 700 6 30 , 600 6 30 , 500 15 75 , 400 6 30 , 300 6 30, 250 15 75, 200 7 35, 150 7 35, 100 7 35, 50 7 35, Total 100 500, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.