Y-27632 Dihydrochloride Solution (10 mM)

Product Introduction Y-27632 is a selective, ATP-competitive inhibitor of ROCK (Rho-associated protein kinase) that is highly potent, cell-permeable, reversible, and highly selective. It demonstrates a substantial ATP-competitive inhibition of p160ROCK (Ki=140 nM) and ROCK-II (IC50=800 nM), as well as a specific inhibitory effect on PRK2 (IC50=600 nM). The addition of Y-27632 to the cell inoculation plate culture is typically made at the initial stage, and it is not required during subsequent medium replacement culture. In stem cell research, Y-27632 (10 µM) treatment of human embryonic stem cells for one hour considerably increased the efficiency of clone creation, prolonged cell passaging ability, and effectively blocked stem cell apoptosis. Furthermore, by controlling processes similar to the epithelial-mesenchymal transition (EMT), Y-27632 was able to specifically drive the differentiation of human induced pluripotent stem cells (hiPSCs) to the mesodermal lineage, offering a crucial tool for research on stem cell-directed differentiation. Common working concentration: 10 µM. Concentration 10 mmol/L Format Liquid Animal Origin Ingredient Without Green Features Sustainable packaging Storage This product can be stored at -5~-20°C for 12 months with shading light. Shipping Ice bag Expiration Date 12 months Matters Need Attention 1. This product was sterilized by 0.1 µm filtration and can be used directly after melting. 2. It is necessary to pay attention to the aseptic operation and avoid the contamination. 3. Before using, the product should be thawed at 2-8°C and shaken thoroughly, repeated freeze-thaw cycles are not advised. 4. If precipitation happens after thawing, the contents can be resuspended by pipetting or vortex mixing. After incubating the solution at 37°C for 20 to 30 minutes or letting it stand at room temperature for about an hour, check to see if the precipitate dissolves as intended. If the product dissolves completely, it can be used as usual. 5. This product is a concentrated solution and should be diluted prior to use as required. 6. The product should be used within a month if stored regularly at 2-8°C. Keep in a frozen state at -5~-20°C for extended storage. Long-term storage at room temperature or between 2-8°C is not recommended. When lesser amounts are required, aliquoting is advised to prevent repeated freeze-thaw cycles. 7. This product is for research use only. 8. Avoiding skin and eye contact. Don't inhale dust, and take relevant precautions when operating.