TYCSB Agar Base (Tryptone Yeast Extract Cystine w/Sucrose) w/ Bacitracin Supplement (supplied separa

Used in the selective isolation of Streptococcus mutans. TYCSB Agar is devised by Gold et al. (1) as a selective medium for Streptococcus mutans with bacitracin and sucrose. Streptococcusmutans is facultatively anaerobic, Gram-positive coccus-shaped bacterium commonly found in the human oral cavity. It is the primary causative agent of dental cavities (2). Conditions in the oral cavity are diverse and complex, frequently changing from one extreme to another. Thus, to survive in the oral cavity, S. mutans must tolerate rapidly harsh environmental fluctuations and exposure to various antimicrobial agents to survive (3). Tryptone and yeast extract in the medium provide nutrients essential for the growth of Streptococci. Sodium sulfite, sodium acetate, disodium phosphate, and sodium bicarbonate are sources of ions that simulate metabolism. Appearance: Cream to yellow homogeneous free flowing powder, Solubility: Light yellow, clear, complete after autoclaving, pH (after autoclaving): 7.1-7.5, Directions per Liter: Dissolve 250 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.