HEK293 Serum-Free Basal Medium

Applicable cell lines: HEK293 serum-free Basal medium is an Animal Derived Component-Free (ADCF) cell culture medium, which is independently developed and produced by the Pricella's R&D team. It can support rapid cell adaptation in a variety of 293 cell lines (such as 293, 293T, 293A and other 293 cells), and has the characteristics of high cell viability, long maintenance time, high transfection efficiency and high yield. Medium characteristics: 1. This product is Animal-Derived Component-Free, ADCF. 2. This product contains amino acids, vitamins, glucose, lipids, inorganic salts, growth factors, hydrolysatesand trace elements, and does not contain peptides. Culture conditions 1. The culture temperature is 37 °C, and the recommended initial cell seeding density is 0.5-1×10^6 cells/mLin batch and fed-batch culture process. 2. Under the condition of non-automatic pH control, it is recommended to control the CO2 concentration at 5%. 3. Process conditions such as pH, DO and temperature can be set according to process development results or platform process parameters.