Dulbecco's MEM (DMEM) w/o Niacinamide, Phenol Red, Sodium Pyruvate (Powder)

Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells. Appearance: White, homogeneous, free-flowing powder Solubility: Colorless, clear, complete pHAs Reported Endotoxi: <1EU/ml Directions: per Liter Dissolve 12.06g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.7g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Storage and Stability: Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.