Useful pH range: 6.5-7.9

Agarose is a fraction extracted from agar-producing seaweeds and it is mainly responsible for the agar's gelling power. It exhibits a high hysteresis (the difference between melting and gelling temperatures) making it ideal for separations such as electrophoresis and chromatography within the fields of Molecular...

SDS is commonly used in the preparation of proteins for electrophoresis in SDS-PAGE. This compound works by disrupting non-covalent bonds in proteins, denaturing them and causing the proteins to lose their natural conformation. The new negative charge is significantly larger than the original charge of that protein....

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100 - 1,500 base pairs. The 500 and 1,500 base pair...

For up to 333 mini gels. The BLUEplus Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and...

MTT, a yellow tetrazole, is reduced to purple formazan in living cells. A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a...

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be...

Recognizes mouse and human NLRP3/NALP3. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the...

Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response...

Recognizes human and mouse Asc. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NALP2, CARD8...

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate...

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate...