Human Sonic Hedgehog (C24II), His-SUMO Tag

Sequence: IIGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS WPGVKLRVTE GWDEDGHHSE ESLHYEGRAV DITTSDRDRS KYGMLARLAV EAGFDWVYYE SKAHIHCSVK AENSVAAKSG with polyhistidine-SUMO tag at the N-terminus Endotoxin level: <0.1 EU per 1 µg of the protein by the LAL method. Protein function: [Sonic hedgehog protein]: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. [The UniProt Consortium]