K-Ras(G12D) Isoform A (human, recombinant)

K-Ras is a small GTPase and member of the RAS family of GTPases with roles in apoptosis, as well as cell proliferation, survival, and migration. K-Ras is composed of a guanine nucleotide-binding domain containing an active site, an effector binding domain, and an isoform-specific C-terminal hypervariable region (HVR) that differs between K-Ras isoforms A and B due to alternative splicing. The active site cycles between GDP-bound inactive and GTP-bound active states and is regulated by its associations with GTPase-activating proteins (GAPs) or guanine nucleotide exchange factors (GEFs). K-Ras is ubiquitously expressed and is tethered to the intracellular side of cell membranes via farnesyl and palmitoyl lipidation and to negatively charged or neutral regions of the membrane via a single polybasic region in the HVR. K-Ras(G12D), which contains a glycine-to-aspartic acid substitution at position 12, is constitutively active and associated with pancreatic, colon, and lung cancers. Inhibition of K-Ras(G12D) with the inhibitory peptide KS-58 reduces tumor volume in a PANC-1 pancreatic cancer mouse xenograft model. Tumor levels of K-Ras(G12D) are increased in patients with lung adenocarcinoma who had never smoked. Cayman's K-Ras(G12D) Isoform A (human, recombinant) protein consists of 185 amino acids and has a calculated molecular weight of 23 kDa.Synonyms: c-K-ras(G12D), c-Ki-ras(G12D), K-Ras4A(G12D), Ki-Ras(G12D), Kirsten Rat Sarcoma Virus(G12D), KRAS(G12D). Purity: >90%. Source: Recombinant human N-terminal His-tagged K-Ras(G12D) isoform A expressed in E. coli. Amino Acids: 2-186. MW: 23 kDa. Formulation: (Request formulation change), 20 mM HEPES, pH 7.4, 150 mM sodium chloride, 10% glycerol, and 1 mM dithiothreitol. UniProt Accession #: P01116.