NFAT Inhibitor

NFAT inhibitor is a peptide inhibitor of the transcription factor nuclear factor of activated T cells (NFAT). It binds to the NFAT recognition site on the protein phosphatase calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity when used at a concentration of 100 µM. NFAT inhibitor inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4 in a concentration-dependent manner. It inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta vascular smooth muscle cells (VSMCs, IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays).Formal Name: L-methionyl-L-alanylglycyl-L-prolyl-L-histidyl-L-prolyl-L-valyl-L-isoleucyl-L-valyl-L-isoleucyl-L-threonylglycyl-L-prolyl-L-histidyl-L-alpha-glutamyl-L-glutamic acid. CAS Number: 249537-73-3. Synonyms: Nuclear Factor of Activated T cells Inhibitor, VIVIT. Molecular Formula: C75H118N20O22S. Formula Weight: 1683.9. Purity: >95%. Formulation: (Request formulation change), A lyophilized powder. Solubility: DMSO: 15 mg/ml, Water: 10 mg/ml. SMILES: O=C(N(CCC1)[C@@H]1C(N[C@@H](C(C)C)C(N[C@]([C@@H](C)CC)([H])C(N[C@@H](C(C)C)C(N[C@]([C@@H](C)CC)([H])C(N[C@]([C@H](O)C)([H])C(NCC(N(CCC2)[C@@H]2C(N[C@H](C(N[C@@H](CCC(O)=O)C(N[C@H](C(O)=O)CCC(O)=O)=O)=O)CC3=CNC=N3)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CC4=CNC=N4)NC([C@H](CCC5)N5C(CNC([C@H](C)NC([C@@H](N)CCSC)=O)=O)=O)=O. InChi Code: InChI=1S/C75H118N20O22S/c1-12-39(7)59(90-70(111)57(37(3)4)88-68(109)52-19-16-27-95(52)74(115)49(30-44-32-78-36-82-44)87-67(108)51-18-15-26-94(51)53(97)33-79-62(103)41(9)83-63(104)45(76)24-28-118-11)72(113)89-58(38(5)6)71(112)91-60(40(8)13-2)73(114)92-61(42(10)96)69(110)80-34-54(98)93-25-14-17-50(93)66(107)86-48(29-43-31-77-35-81-43)65(106)84-46(20-22-55(99)100)64(105)85-47(75(116)117)21-23-56(101)102/h31-32,35-42,45-52,57-61,96H,12-30,33-34,76H2,1-11H3,(H,77,81)(H,78,82)(H,79,103)(H,80,110)(H,83,104)(H,84,106)(H,85,105)(H,86,107)(H,87,108)(H,88,109)(H,89,113)(H,90,111)(H,91,112)(H,92,114)(H,99,100)(H,101,102)(H,116,117)/t39-,40-,41-,42+,45-,46-,47-,48-,49-,50-,51-,52-,57-,58-,59-,60-,61-/m0/s1. InChi Key: QPMHUXBSHGAVGD-MCDIZDEASA-N.