Ac-LEVD-AFC

Ac-LEVD-AFC is a fluorogenic substrate for caspase-4. Upon enzymatic cleavage by caspase-4, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-4 activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.Formal Name: N-acetyl-L-leucyl-L-alpha-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine. CAS Number: 1092077-23-0. Synonyms: Ac-Leu-Glu-Val-Asp-AFC, Ac-Leu-Glu-Val-Asp-7-amino-4-trifluormethylcoumarin. Molecular Formula: C32H40F3N5O11. Formula Weight: 727.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 234, 340 nm. SMILES: CC(N[C@@H](CC(C)C)C(N[C@@H](CCC(O)=O)C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O)=O)=O)=O)=O. InChi Code: InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)/t20-,21-,22-,27-/m0/s1. InChi Key: FETZKXHXYOHAAN-LFYAFONDSA-N.