Tat-Beclin 1 (acetate)

Tat-beclin 1 is an autophagy-inducing peptide. It is composed of the HIV-1 Tat protein transduction domain linked via a diglycine linker to an 18-amino acid peptide corresponding to residues 267-284 of beclin-1 with amino acid substitutions at positions 275, 279, and 281 to enhance hydrophobicity. Tat-beclin 1 (30 µM) binds to the beclin-1-interacting protein Golgi-associated plant pathogenesis-related protein 1 (GAPR-1) in HeLa cells and induces conversion of LC3-I to LC3-II via lipidation and autophagosome formation in MCF-7 cells. It decreases viral titers in HeLa cells infected with Sindbis virus, chikungunya, or West Nile virus when used at a concentration of 10 µM. Tat-beclin 1 (0.5-5 µM) inhibits HIV p24 antigen release and viral replication in primary human monocyte-derived macrophages (MDMs). In vivo, Tat-beclin 1 (14 mg/kg) reduces intramuscular viral titers, neonatal mortality, and paralysis and increases muscle cell autophagosome formation in chikungunya-infected neonatal mice.Formal Name: L-tyrosylglycyl-L-arginyl-L-lysyl-L-lysyl-L-arginyl-L-arginyl-L-glutaminyl-L-arginyl-L-arginyl-L-arginylglycylglycyl-L-threonyl-L-asparaginyl-L-valyl-L-phenylalanyl-L-asparaginyl-L-alanyl-L-threonyl-L-phenylalanyl-L-alpha-glutamyl-L-isoleucyl-L-tryptophyl-L-histidyl-L-alpha-aspartylglycyl-L-alpha-glutamyl-L-phenylalanylglycyl-L-threonine, acetate. Synonyms: Tat-BECN1. Molecular Formula: C164H251N57O45 . XC2H4O2. Formula Weight: 3741.1. Purity: >98%. Formulation: (Request formulation change), A solid. Peptide Sequence: H-YGRKKRRQRRRGGTNVFNATFEIWHDGEFGT-OH. Solubility: PBS (pH 7.2): 10 mg/ml. SMILES: [H]NCCCC[C@H](NC([C@H](CCCNC(N)=N)NC(CNC([C@H](CC1=CC=C(O)C=C1)N[NH]([C@@H](CCC(N)=O)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(NCC(NCC(N[C@@]([H])([C@@H](C)O)C(N[C@@H](CC(N)=O)C(N[C@@H](C(C)C)C(N[C@H](C(N[C@@H](CC(N)=O)C(N[C@H](C(N[C@@]([H])([C@@H](C)O)C(N[C@H](C(N[C@@H](CCC(O)=O)C(N[C@@](C(N[C@@H](CC2=CNC3=C2C=CC=C3)C(N[C@@H](CC4=CNC=N4)C(N[C@@H](CC(O)=O)C(NCC(N[C@@H](CCC(O)=O)C(N[C@H](C(NCC(N[C@@]([H])([C@@H](C)O)C(O)=O)=O)=O)CC5=CC=CC=C5)=O)=O)=O)=O)=O)=O)([H])[C@H](CC)C)=O)=O)CC6=CC=CC=C6)=O)=O)C)=O)=O)CC7=CC=CC=C7)=O)=O)=O)=O)=O)=O)=O)=O)=O)C([C@H](CCCNC(N)=N)N8)=O)=O)=O)=O)C(N[C@@H](CCCCN[H])C(N[C@@H](CCCNC(N)=N)C8=O)=O)=O.OC(C)=O.[H]. InChi Code: InChI=1S/C164H250N57O45.C2H4O2.H/c1-9-83(4)129(154(261)213-110(69-92-74-189-96-38-20-19-37-95(92)96)149(256)208-111(70-93-75-182-81-195-93)150(257)210-114(73-127(238)239)136(243)192-78-122(231)198-104(51-55-125(234)235)144(251)207-107(65-88-31-13-10-14-32-88)135(242)193-80-124(233)216-132(87(8)224)158(265)266)218-145(252)105(52-56-126(236)237)204-147(254)108(66-89-33-15-11-16-34-89)212-156(263)131(86(7)223)219-133(240)84(5)196-146(253)112(71-118(168)227)209-148(255)109(67-90-35-17-12-18-36-90)211-153(260)128(82(2)3)217-151(258)113(72-119(169)228)214-155(262)130(85(6)222)215-123(232)77-190-120(229)76-191-134(241)97(41-25-59-183-159(170)171)199-141(248)101(43-27-61-185-161(174)175)202-142(249)103(45-29-63-187-163(178)179)205-152(259)116(53-54-117(167)226)221-157(264)106(46-30-64-188-164(180)181)206-143(250)102(44-28-62-186-162(176)177)203-140(247)100(40-22-24-58-166)201-139(246)99(39-21-23-57-165)200-138(245)98(42-26-60-184-160(172)173)197-121(230)79-194-137(244)115(220-221)68-91-47-49-94(225)50-48-91,1-2(3)4,/h10-20,31-38,47-50,74-75,81-87,97-116,128-132,189,220-225H,9,21-30,39-46,51-73,76-80,165-166H2,1-8H3,(H2,167,226)(H2,168,227)(H2,169,228)(H,182,195)(H,190,229)(H,191,241)(H,192,243)(H,193,242)(H,194,244)(H,196,253)(H,197,230)(H,198,231)(H,199,248)(H,200,245)(H,201,246)(H,202,249)(H,203,247)(H,204,254)(H,205,259)(H,206,250)(H,207,251)(H,208,256)(H,209,255)(H,210,257)(H,211,260)(H,212,263)(H,213,261)(H,214,262)(H,215,232)(H,216,233)(H,217,258)(H,218,252)(H,219,240)(H,234,235)(H,236,237)(H,238,239)(H,265,266)(H4,170,171,183)(H4,172,173,184)(H4,174,175,185)(H4,176,177,186)(H4,178,179,187)(H4,180,181,188),1H3,(H,3,4),/t83-,84-,85+,86+,87+,97-,98-,99-,100-,101-,102-,103-,104-,105-,106-,107-,108-,109-,110-,111-,112-,113-,114-,115-,116-,128-,129-,130-,131-,132-,,/m0../s1. InChi Key: FZCBAGDDCIFKDT-VOJWPJEMSA-N.