Protein Kinase R, pT451 non-phosphorylated Peptide (PKR, ds RNA-dependent Protein Kinase)

Negative control peptide for the PKR phosphothreonine 451 rabbit polyclonal antibody. Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation. Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at Thr446 and Thr451 in the activation loop. Substitution of Thr451 to alanine completely inactivates PKR, while a mutant with a Thr446 to alanine substitution was partially active. PKR phosphorylates the a subunit of eukaryotic initiation factor-2 and inhibits protein synthesis. It is an important component of the interferon response to virus infection. Recently, it has been reported that PKR also phosphorylates human p53 on serine392. , Storage and Stability: , Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.