GW 5074

Raf-1 is a proto-oncogene serine/threonine protein kinase that signals from Ras to the MAPK/ERK signaling pathway. This pathway mediates basic cellular functions, including proliferation, differentiation, and survival. GW 5074 is a potent, selective, and cell-permeable inhibitor of Raf-1 (IC50 = 9 nM). It blocks phosphorylation of ERK1/2 by 90% in cells stimulated with epidermal growth factor when given at 5 µM. GW 5074 shows more than 100-fold selectivity for Raf-1 versus several related kinases.Formal Name: 3-[(3,5-dibromo-4-hydroxyphenyl)methylene]-1,3-dihydro-5-iodo-2H-indol-2-one. CAS Number: 220904-83-6. Molecular Formula: C15H8Br2INO2. Formula Weight: 520.9. Purity: >98% (mixture of cis and trans). Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 15 mg/ml, DMSO: 15 mg/ml, DMSO:PBS (pH 7.2)(1:8): 0.1 mg/ml. lambdamax: 260, 348 nm. SMILES: IC1=CC=C(NC(/C2=C\C3=CC(Br)=C(O)C(Br)=C3)=O)C2=C1. InChi Code: InChI=1S/C15H8Br2INO2/c16-11-4-7(5-12(17)14(11)20)3-10-9-6-8(18)1-2-13(9)19-15(10)21/h1-6,20H,(H,19,21)/b10-3-. InChi Key: LMXYVLFTZRPNRV-KMKOMSMNSA-N.