Bucculite(TM) dT Incorporation Cell Proliferation Fluorescence Imaging Kit *Deep Red Fluorescence*

Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Buccutite(TM) dT Incorporation Cell Proliferation Assay Kit uses FOL-dU, an analog of thymidine. FOL-dU is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated FOL-dT is labelled with MTA-iFluor(TM) 647 through our Buccutite(TM) labeling technology. The resulted iFluor(TM) 647-labeled DNA formed in cells is visualized in Cy5 Channel. Buccutite(TM) dT Incorporation Cell Proliferation Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry assay. It is an environment friendly coper-free assay for measuring active DNA synthesis at single-cell level.