S-Glutathionylated Protein Detection Kit

S-Glutathionylated Protein Detection Kit
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
Cay10010721-1 1 each -

1 - 10 Werktage

375,00 €
Glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) widely distributed in both... mehr
Produktinformationen "S-Glutathionylated Protein Detection Kit"
Glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) widely distributed in both plants and animals. GSH is involved in maintenance of protein sulfhydryl reduction status. The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver. Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest. Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the "Biotin-Switch" method. Cayman's S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.
Hersteller: Cayman Chemical
Hersteller-Nr: 10010721-1


Anwendung: S-Glutathionylated Protein Detection
Format: Homog.

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: -20°C (International: -20°C)
Signalwort: Danger
H-Sätze: H302, H311, H314, H315, H317, H318, H319, H332, H335
P-Sätze: P260, P261, P264, P270, P271, P272, P280, P312, P321, P363, P301+P310, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P305+P351+P338, P332+P313, P333+P313, P337+P313, P361+P364, P362+P364, P405, P403+P233, P501
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