Cell Glutathione Peroxidase (GPX) Activity Assay Kit

Detection principle: GPX catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents. The reducing agent has a maximum absorbance at 340 nm. The activity of GPX can be calculated by measuring the change of absorbance value at 340 nm. Performance characteristics: Sample type: cell. Sensitivity: 3.22 U/L. Detection range: 3.22-446.95 U/L. Detection method: Colorimetric method. Assay type: Enzyme Activity. Assay time: 30 min. Precision: Average inter-assay CV: 0.4-3.4%, Average intra-assay CV: 1.2-3.1%. Other reagents required: Double distilled water, Normal saline (0.9% NaCl). Storage: -20?. Valid period: 12 months. Protein function: Catalyzes the reduction of hydroperoxides in a glutathione- dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402). [The UniProt Consortium]