TrkA BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human trkA in cell culture supernates, cell lysates and tissue homogenates. Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain. TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor. TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in kehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor. The standard product used in this kit is extracellular part (A33-P418) of recombinant human TRKA, consisting of 386 amino acids with the molecular weight of 69kD. , Assay Range:, 156pg/ml-10000pg/ml, Sensitivity: < 10 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P04629, Entrez Gene ID: 4914, Kit Components: 96-well plate pre-coated with anti-TrkA, Human, Lyophilized recombinant TrkA, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.