Anti-Phosphoserine, clone 3C171

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. Applications: Suitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot (Indirect): 1:500-1:1000, Indirect ELISA: 1:8000-1:16,000, Note: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead., Optimal dilutions to be determined by researcher. Positive Control: Rat brain cortex and extract at ~50kD. Hybridoma: Mouse myeloma cells with splenocytes from immunized mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.