Anti-phospho-Src (Tyr418) (pp60Src, pp60c-Src)

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Applications: Suitable for use in Western Blot, Immunocytochemistry, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000. Detects phospho-Src (Tyr418) in RIPA lysate of E16 rat heart. , Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.