Anti-phospho-Protein Kinase C, alpha/beta2, (Thr638/641) (PKC-b2)

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising alpha, betaI (also known as beta1), betaII (also known as beta2) and gamma)), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation, novel (n)PKC isoforms (comprising delta, epsilon, eta (also known as PKC-L), theta and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+, and atypical (a)PKC isoforms, namely zeta, iota and lambda (the mouse homolog of human PKC iota) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCnu. , PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. , PKC activity is regulated through three distinct phosphorylation sites, the activation loop threonine (Thr497 in PKCalpha and Thr500 in PKCbeta2), the turn loop autophosphorylation threonine (Thr638 in PKCalpha and Thr641 in PKCbeta2) and the hydrophobic loop serine (Ser657 in PKCalpha and Ser660 in PKCbeta2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.