Anti-phospho-GATA4 (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4)

GATA-4 is a 46kD member of the GATA family of zinc finger-containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, it plays a critical role in regulating basal and agonist or stress-induced gene expression in cardiac and smooth muscle cell types. GATA-4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA-4 is phosphorylated in response to agonist stimulation through MEK1-ERK1/2 and weakly through JNK or p38 MAPKs. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml, Optimal dilutions to be determined by the researcher. Positive Controls Used: Phenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA-4. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.