Anti-phospho-EphrinB (Thr298)

Anti-EphrinB pT298 antibody is directed againts rat Ephrin. Anti-EphrinB pT298 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated EphrinB. Minimal reactivity occurs against non-phosphorylated EphrinB pT298. Reactivity is completely blocked by treatment with ?-phosphatase. Reactivity against EphrinB occurs from chicken and rat sources. However, reactivity is also expected against the following species based on 100% sequence homology: bovine, canine, human, mouse, NHP, Xenopus and zebra fish. Protein function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Beside axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. [The UniProt Consortium]