Anti-Histone H3K9Me3/S10Ph Monoclonal Antibody (RM162)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Trimethylation of lysine 9 and phosphorylation of serine 10 on histone H3 (H3K9Me3/S10Ph) is associated with repression of polycomb-regulated genes in differentiated mesenchymal stem cells (MSCs) and resting B cells but not pluripotent embryonic stem cells (ESCs). Cayman's H3K9Me3/S10Ph Monoclonal Antibody (RM162) recognizes histone H3 only when modified by both trimethylation at K9 and phosphorylation at S10. This antibody can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.Immunogen: Peptide corresponding to H3K9Me3/S10Ph. Clone Designation: RM162. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H3K9Me3/S10Ph, (-) Unmodified histone H3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3S10Ph.