Anti-CaMKII (Phospho-Thr286)

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine protein kinase and member of the calcium/calmodulin-activated protein kinase family. There are four isoforms of CaMKII, alpha, beta, gamma, and delta, encoded by CaMK2A, CaMK2B, CaMK2G, and CaMK2D in humans. CaMKIIalpha and CaMKIIbeta are predominantly expressed in neuronal tissue, while CaMKIIgamma and CaMKIIdelta are primarily found in cardiac tissue, however, the various isoforms of CaMKII can be found in nearly all cell types. Upon calcium binding, CaMKII undergoes a conformational switch that displaces the autoinhibitory domain inducing autophosphorylation of threonine 286 (Thr286) and autonomous activation of the kinase. Activation of CaMKII in dendritic spines in response to increased calcium concentration facilitates the induction of long term potentiation (LTP) and memory formation in rodents. Phospho-site mutant Camk2aT286A knock-in mice exhibit impaired LTP, as well as learning and memory deficits. Cayman's CaMKII (Phospho-Thr286) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes CaMKIIalpha phospho-Thr286 and CaMKIIbeta phospho-Thr286 at approximately 50 and 60 kDa, respectively, from human, mouse, rabbit, and rat samples.Synonyms: Calcium/Calmodulin-dependent Protein Kinase II. Immunogen: Phosphopeptide corresponding to amino acid residues surrounding phospho-Thr286 of rat CaM Kinase II. MW: ~50 and 60 kDa for CaMKIIalpha phospho-Thr286 and CaMKIIbeta phospho-Thr286, respectively. Formulation: (Request formulation change), 100 µl of Affinity-purified rabbit polyclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC, WB.