Anti-Acetyl-CoA Carboxylase 1 (Phospho-Ser79) Rabbit Monoclonal Antibody (Clone RM270)

Acetyl-CoA carboxylase 1 (ACC1) is a biotin-dependent enzyme that catalyzes the conversion of acetyl-CoA (Cay-16160) to malonyl-CoA (Cay-16455), a building block in the biosynthesis of long-chain fatty acids. It is found at high levels in the liver and adipose tissue and is localized to the cytosol. Expression of ACC1 is increased by sterol regulatory element binding protein-1a (SREBP-1a) and SREBP-1c, which are induced by insulin, as well as the lipid precursors inositol and choline (Cay-31178). ACC1 activity is increased by citrate and inhibited by binding to long-chain acyl-CoAs or by phosphorylation by a number of kinases, including AMPK and TAK1. Phosphorylation of ACC1 at serine 79 by AMPK inhibits the catalytic activities of ACC1, preventing the generation of malonyl-CoA, whereas dephosphorylation by protein phosphatase 4 promotes hepatic lipogenesis. Knock-in mice with an alanine in place of serine at position 79 to prevent phosphorylation at this residue of Acc1 have increased de novo lipogenesis, reduced beta-oxidation, and increased insulin resistance and glucose intolerance. Cayman's Acetyl-CoA Carboxylase 1 (Phospho-Ser79) Rabbit Monoclonal Antibody (Clone RM270) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: ACCalpha (Phospho-Ser79), pACC1-Ser79, pSer79 ACC1. Immunogen: Peptide corresponding to human ACC1 (phospho-Ser79). Clone Designation: RM270. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) ACC1 (phospho-Ser79), (-) ACC1 without phosphorylation at Ser79.