The His-tagged expression system is widely used in the production of recombinant proteins. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:1000, Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, I1904-65C: IgG, H&L (HRP) Pab Rb x Mo, I1904-65H: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Mo, I1904-65N: IgG (HRP) Pab Rb x Mo, I1903-07E: IgG (HRP) Pab Rb x Mo, I1904-67R: IgG H&L, X-adsorbed (HRP) Pab Rb x Mo, Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.