Anti-MBP Nanobody Affinity Agarose

Coupled ligand: Anti-MBP nanobody. Beads: Sepharose 4B agarose with average particle size of 50 µm. Binding capacity: 1.2mg protein /ml agarose. Storage buffer: PBS with preservative and 50% glycerol, pH7.2. Storage conditions: -20? for 12 months. Background: MBP (Maltose Binding Protein) is a commonly used affinity tag, which can increase the expression level and solubility of the target protein. Anti-MBP affinity gel is made by covalently coupling high-quality MBP nanobody with agarose gel. Since the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of MBP tagged fusion protein.