Collagenase/Elastase

Collagenase/Elastase
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
MB-126-0001 1 each - -

6 - 10 Werktage*

200,00 €
 
Collagenase/Elastase is partially purified and 0.22µm filtered and contains 22,500 units of... mehr
Produktinformationen "Collagenase/Elastase"
Collagenase/Elastase is partially purified and 0.22µm filtered and contains 22,500 units of collagenase type 1 and 30 units of elastase per vial. Collangenase type 1 has the original balance of collagenase, caseinase, clostripain and tryptic activities. The collagenase assay is a modification of the Mandl collagen digestion procedure wherein collagenase is incubated for five hours with native collagen and the extent of collagen breakdown is determined using the Moore and Stein, JBC, 176, 367, (1948) colorimetric ninhydrin method. Amino acids released are expressed as micromoles L-leucine per milligram collagenase in 5 hours at 37°C, pH 7.5. Caseinase activity, a measure of non-specific proteolytic activity, is determined using the above assay and substituting 25 milligrams vitamin free casein for the collagen substrate. Caseinase activity is calculated as for collagenase activity. Clostripain activity is measured after activation in 2.5 mM dithiothreitol (DTT). One unit hydrolyzes one micromole of BAEE per minute at 25°C, pH 7.6, after activation. Tryptic activity is assayed using the same BAEE method as clostripain, but without activation. Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. Suitable for the isolation of Type II lung cells. Protein function: Acts upon elastin. [The UniProt Consortium]
Schlagworte: ELA1, CELA1, Elastase-1, EC=3.4.21.36, Chymotrypsin-like elastase family member 1
Hersteller: Rockland Immunochemicals
Hersteller-Nr: MB-126-0001

Eigenschaften

Konjugat: No

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +20°C (International: +20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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