Anti-Von Willebrand Factor (VWF, F8VWF)

Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein. It has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. As not all endothelial cells synthesize / store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence. Applications: Suitable for use in ELISA, In immunoelectrophoresis, Bi-dimensional Electrophoresis and , Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum, Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm, diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter, peripheral wells., Single Radial Immunodiffusion: 1% in the gel., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.