Anti-Vinculin/VCL (Metavinculin)

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is , involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a , 3-generation family, Mulligan et al. (1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to , D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is , thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. , Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity , of some variants has not been determined. Human vinculin protein exhibits a greater-than-95% , sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 , amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain , separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains , binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region , contains binding sites for F-actin, paxillin, and lipids. UniProt Number: P18206, Gene ID: VCL, Applications: Suitable for use in Western Blot and Immunohistochemistry (Paraffin). Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.